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B(12)-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics.
Yang Z, Fok HKF, Luo J, Yang Y, Wang R, Huang X, Sun F
Sci Adv. 2022 Apr;8(13):eabm5482. doi: 10.1126/sciadv.abm5482. Epub 2022 Apr 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196474p32m3C Trx-CNProtein expression of Thiolredoxin-3C cleavage site-CarHCN
196475p32m3C Trx-CCProtein expression of Thiolredoxin-3C cleavage site-CarHCC
196477pQE80l B-CN-BProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
196478pQE80l A-CC-AProtein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

Antibodies from Article