Addgene: Site-specific incorporation of unnatural amino acids into proteins in mammalian cells.

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Site-specific incorporation of unnatural amino acids into proteins in mammalian cells.
Hino N, Sakamoto K, Yokoyama S
Methods Mol Biol. 2012;794:215-28. doi: 10.1007/978-1-61779-331-8_13. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
196483	pcpAzpaRS_v2	To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
196484	pcpBpaRS	To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
196485	pcpBpaRS_v2	To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
196486	pcPylRS(Y384F Y306A)	To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

Antibodies from Article