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Synthetic regulatory genomics uncovers enhancer context dependence at the Sox2 locus.
Brosh R, Coelho C, Ribeiro-Dos-Santos AM, Ellis G, Hogan MS, Ashe HJ, Somogyi N, Ordonez R, Luther RD, Huang E, Boeke JD, Maurano MT
Mol Cell. 2023 Apr 6;83(7):1140-1152.e7. doi: 10.1016/j.molcel.2023.02.027. Epub 2023 Mar 16. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196992pLP-PIGA3 (pLP305)Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
196993pRB051_LEU2Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Antibodies from Article