Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Stomach-derived human insulin-secreting organoids restore glucose homeostasis.
Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q
Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y. PubMed Article

Plasmids from Article

ID Plasmid Purpose
197385pLenti-EF1a-Ngn3ER-PuroR-mCherryTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)
197386pLenti-CMV-PMTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).
197387pLenti-TetO-PMTo establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Antibodies from Article