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Stomach-derived human insulin-secreting organoids restore glucose homeostasis.
Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q
Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
197385pLenti-EF1a-Ngn3ER-PuroR-mCherryTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)
197386pLenti-CMV-PMTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).
197387pLenti-TetO-PMTo establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

Antibodies from Article