Addgene: Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant.

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Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant.
Dema A, Charafeddine R, Rahgozar S, van Haren J, Wittmann T
Elife. 2023 Jan 30;12:e84143. doi: 10.7554/eLife.84143. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
197419	pEB1N-mScarlet-LZ-LOV2	Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1
197420	pSpCas9(BB)-2A-mCherry	Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA
197421	pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1	Knock out of EB3 in human cells by CRISPR/Cas9
197422	pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2	Knock out of EB3 in human cells by CRISPR/Cas9
197423	pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3	Knock out of EB3 in human cells by CRISPR/Cas9
197424	pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1	π-element knock-in into exon 5 of MAPRE1
197425	pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2	π-element knock-in into exon 5 of MAPRE1
197426	pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3	π-element knock-in into exon 5 of MAPRE1
197427	pUC19-π-EB1_IRES-mCherry	Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5
197429	pUC19-π-EB1_EF1α-mCherry	Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
197430	pUC19-π-EB1_EF1α-EGFP	Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

Antibodies from Article