RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science. 2022 Nov 25;378(6622):874-881. doi: 10.1126/science.add7450. Epub 2022 Nov 3. PubMed Article
Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
Science. 2022 Nov 25;378(6622):874-881. doi: 10.1126/science.add7450. Epub 2022 Nov 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197603 | pCDNA3_Chrm3_Csx30_Cre | Cre reporter for mammalian cells with Chrm3 membrane anchor |
| 197604 | pCDNA3_Gap43_Csx30_Cre | Cre reporter for mammalian cells with Gap43 membrane anchor |
| 197605 | pCDNA3_dCas7-11 | Expresses inactive Cas7-11 in mammalian cells |
| 197606 | pCDNA3_Csx29 | Expresses Csx29 in mammalian cells |
| 197607 | pCDNA3_Cas7-11_T2A_Csx29 | Expresses Cas7-11 and Csx29 in mammalian cells |
| 197609 | U6_crRNA_mCherry | Expresses a Cas7-11 crRNA and mCherry |
| 197610 | pCDNA3_HA_Csx30 | Expresses a HA-tagged version of Csx30 |
| 197611 | SUMO_TwinStrep_Csx30 | For protein purification of Csx30 |
| 197612 | SUMO_TwinStrep_Cas7-11_crRNA | For protein purification of Cas7-11 |
| 197613 | pCDF_6xHIS_Csx29 | For protein purification of Csx29 |
| 197614 | pCDF_HA_CASP_sigma | For expression of HA-tagged CASP sigma in E. coli |