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Unmyelinated sensory neurons use Neuregulin signals to promote myelination of interneurons in the CNS.
Lysko DE, Talbot WS
Cell Rep. 2022 Nov 15;41(7):111669. doi: 10.1016/j.celrep.2022.111669.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
197647zCREST3:Cas9green; T2_gRNApDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA
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199332zCREST3:Cas9green; T1_gRNApDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA
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199333zCREST3:Cas9green; EGF_gRNApDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA
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199334zCREST3:redCas9; control_gRNApDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA
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199335zCREST3:Cas9green; T3_gRNApDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA
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199336pTol2_claudink:Cas9green; control_gRNApDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA
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199337pTol2_NBT:Cas9green; erbb2_gRNA171pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA
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199338pTol2_LSEC:Cas9green; erbb2_gRNA171pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA
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199339pTol2_claudink:Cas9green; erbb2_gRNA171pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA
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199341p3E_afp_UTRpAMS448; Gateway 3' entry clone encoding ocean pout antifreeze (afp) protein 3'UTR
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199385pDest_U6_A2_CG2_erbb2_gRNA171pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA
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199386pDest_U6_A2_CG2_typeEGF_gRNApDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA
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199387pDest_U6_A2_CG2_T1_gRNApDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNA
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199388pDest_U6_A2_CG2_T2_gRNApDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA
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199389pDest_U6_A2_CG2_T3_gRNApDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA
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199393p5E_(-3kb)nrg1 type IIpDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence
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199394pTol2_(-3kb)nrg1_typeII:GFP-CAAXpDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control
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199395p5E_ngn1_LSE_CpDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence
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Antibodies from Article