Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV production
Jain NK, Ogden PJ, Church GM
bioRxiv 2023.01.31.526541 Article
Jain NK, Ogden PJ, Church GM
bioRxiv 2023.01.31.526541 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198014 | pCMV-AAV2cap | For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198015 | pCMV-AAV5cap | For high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198016 | pCMV-AAV9cap | For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198050 | pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) | By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes |