Eric Davis Lab:
Transposon vectors for proximity biotin labeling with split TurboID
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198217 | pSBtet-Bla/PAG1-TurboID-GAI | Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling |
| 198218 | pSBtet-Bla/mCardinal-TurboID-GAI | Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling |
| 198219 | pSBbi-Pur/GDI1-splitTurboID | Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling |
| 198220 | pSBtet-Bla/PAG1-GFP | Transposon vector (Addgene #60510) for doxycycline-inducible expression of human PAG1 fused to EGFP |
| 198221 | pSBtet-Bla/GFP | Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP |
| 198222 | pHR_SFFV*/Puro2a-GFP | Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP |
| 198223 | pHR_SFFV*/Puro2a-PAG1-GFP | Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP |