Glow-in-the-Dark Infectious Disease Diagnostics Using CRISPR-Cas9-Based Split Luciferase Complementation.
van der Veer HJ, van Aalen EA, Michielsen CMS, Hanckmann ETL, Deckers J, van Borren MMGJ, Flipse J, Loonen AJM, Schoeber JPH, Merkx M
ACS Cent Sci. 2023 Mar 15;9(4):657-667. doi: 10.1021/acscentsci.2c01467. eCollection 2023 Apr 26. PubMed Article
van der Veer HJ, van Aalen EA, Michielsen CMS, Hanckmann ETL, Deckers J, van Borren MMGJ, Flipse J, Loonen AJM, Schoeber JPH, Merkx M
ACS Cent Sci. 2023 Mar 15;9(4):657-667. doi: 10.1021/acscentsci.2c01467. eCollection 2023 Apr 26. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198750 | pET28a(+)-SpdCas9-SBiT_Strep-Tag-II | Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase |
| 198751 | pET28a(+)-SpdCas9-LBiT_Strep-Tag-II | Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase |