GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins.
Thames AH, Moons SJ, Wong DA, Boltje TJ, Bochner BS, Jewett MC
ACS Synth Biol. 2023 Apr 11. doi: 10.1021/acssynbio.3c00017. PubMed Article
Thames AH, Moons SJ, Wong DA, Boltje TJ, Bochner BS, Jewett MC
ACS Synth Biol. 2023 Apr 11. doi: 10.1021/acssynbio.3c00017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199113 | CSTI.pJL1 | In vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrate |
| 199115 | NeuA.pJL1 | In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1) |
| 199116 | Derp2.pJL1 | In vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT) |