A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria
Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
bioRxiv 2023.05.19.541510 Article
Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
bioRxiv 2023.05.19.541510 Article
These plasmids are available to request as a set: Cultivarium POSSUM Toolkit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198918 | pGL0_1 [RK2 ORI] | Level 0 part |
| 198919 | pGL0_2 [pBBR1 ORI] | Level 0 part |
| 198920 | pGL0_3 [RSF1010 ORI] | Level 0 part |
| 198921 | pGL0_4 [pIJ101 ORI] | Level 0 part |
| 198922 | pGL0_5 [pAL5000 ORI] | Level 0 part |
| 198923 | pGL0_7 [pSa ORI] | Level 0 part |
| 198924 | pGL0_9 [pUB110 ORI] | Level 0 part |
| 198925 | pGL0_11 [pWKS1 ORI] | Level 0 part |
| 198926 | pGL0_12 [pAMβ1 ORI] | Level 0 part |
| 198927 | pGL0_13 [pIP501 ORI] | Level 0 part |
| 198928 | pGL0_15 [CEN6/ARSH4 ORI] | Level 0 part |
| 198929 | pGL0_16 [2μ ORI] | Level 0 part |
| 198930 | pGL0_17 [AMA1 short ORI] | Level 0 part |
| 198931 | pGL0_18 [AMA1 long ORI] | Level 0 part |
| 198932 | pGL0_19 [panARS ORI] | Level 0 part |
| 198933 | pGL0_20 [panARS-opt ORI] | Level 0 part |
| 198934 | pGL0_21 [UARS1 ORI] | Level 0 part |
| 198935 | pGL0_22 [pSG5 ORI] | Level 0 part |
| 198936 | pGL0_23 [pSC101ts ORI] | Level 0 part |
| 198937 | pGL0_24 [pBBR1-UP ORI] | Level 0 part |
| 198938 | pGL0_25 [P3 promoter] | Level 0 part |
| 198939 | pGL0_26 [bla pBR322] | Level 0 part |
| 198940 | pGL0_27 [Tfd gene VIII] | Level 0 part |
| 198941 | pGL0_28 [Paph(3')-I EZ-Tn5] | Level 0 part |
| 198942 | pGL0_29 [aph(3')-I EZ-Tn5] | Level 0 part |
| 198943 | pGL0_30 [PaphA-3 ] | Level 0 part |
| 198944 | pGL0_31 [aphA-3] | Level 0 part |
| 198945 | pGL0_32 [Pcat] | Level 0 part |
| 198946 | pGL0_33 [cat] | Level 0 part |
| 198947 | pGL0_34 [PaadA] | Level 0 part |
| 198948 | pGL0_35 [aadA] | Level 0 part |
| 198949 | pGL0_36 [PtetA pSC101] | Level 0 part |
| 198950 | pGL0_37 [tetA pSC101] | Level 0 part |
| 198951 | pGL0_38 [Paac(3)-Ia] | Level 0 part |
| 198952 | pGL0_39 [aac(3)-Ia] | Level 0 part |
| 198953 | pGL0_40 [dfrA1] | Level 0 part |
| 198954 | pGL0_41 [Paac3-IV] | Level 0 part |
| 198955 | pGL0_42 [aac3-IV] | Level 0 part |
| 198956 | pGL0_43 [tsr] | Level 0 part |
| 198957 | pGL0_44 [hph] | Level 0 part |
| 198958 | pGL0_45 [nat1] | Level 0 part |
| 198959 | pGL0_46 [nptII] | Level 0 part |
| 198960 | pGL0_47 [bar] | Level 0 part |
| 198961 | pGL0_48 [ble] | Level 0 part |
| 198962 | pGL0_49 [tk101] | Level 0 part |
| 198963 | pGL0_50 [catE] | Level 0 part |
| 198964 | pGL0_51 [PcatA] | Level 0 part |
| 198965 | pGL0_52 [catA] | Level 0 part |
| 198966 | pGL0_53 [TcatA] | Level 0 part |
| 198967 | pGL0_54 [PermC] | Level 0 part |
| 198968 | pGL0_55 [ermC] | Level 0 part |
| 198969 | pGL0_56 [TermC] | Level 0 part |
| 198970 | pGL0_57 [PtetL] | Level 0 part |
| 198971 | pGL0_58 [tetL] | Level 0 part |
| 198972 | pGL0_59 [TtetL] | Level 0 part |
| 198973 | pGL0_60 [sfGFP] | Level 0 part |
| 198974 | pGL0_62 [sfYFP] | Level 0 part |
| 198975 | pGL0_64 [mScarlet] | Level 0 part |
| 198976 | pGL0_65 [StayGold] | Level 0 part |
| 198977 | pGL0_76 [ORI_BC_1] | Level 0 part |
| 198978 | pGL0_77 [ORI_BC_2] | Level 0 part |
| 198979 | pGL0_78 [ORI_BC_3] | Level 0 part |
| 198980 | pGL0_79 [ORI_BC_4] | Level 0 part |
| 198981 | pGL0_80 [ORI_BC_5] | Level 0 part |
| 198982 | pGL0_81 [ORI_BC_6] | Level 0 part |
| 198983 | pGL0_82 [ORI_BC_7] | Level 0 part |
| 198984 | pGL0_83 [ORI_BC_8] | Level 0 part |
| 198985 | pGL0_84 [ORI_BC_9] | Level 0 part |
| 198986 | pGL0_85 [ORI_BC_10] | Level 0 part |
| 198987 | pGL0_86 [ORI_BC_11] | Level 0 part |
| 198988 | pGL0_87 [ORI_BC_12] | Level 0 part |
| 198989 | pGL0_88 [ORI_BC_13] | Level 0 part |
| 198990 | pGL0_89 [ORI_BC_14] | Level 0 part |
| 198991 | pGL0_90 [ORI_BC_15] | Level 0 part |
| 198992 | pGL0_91 [ORI_BC_16] | Level 0 part |
| 198993 | pGL0_92 [ORI_BC_17] | Level 0 part |
| 198994 | pGL0_93 [ORI_BC_18] | Level 0 part |
| 198995 | pGL0_94 [ORI_BC_19] | Level 0 part |
| 198996 | pGL0_95 [ORI_BC_20] | Level 0 part |
| 198997 | pGL0_96 [J23100] | Level 0 part |
| 198998 | pGL0_97 [J23108] | Level 0 part |
| 198999 | pGL0_98 [J23114] | Level 0 part |
| 199000 | pGL0_99 [bsRBS] | Level 0 part |
| 199001 | pGL0_100 [Pbs] | Level 0 part |
| 199002 | pGL0_101 [PnptII] | Level 0 part |
| 199003 | pGL0_102 [pAgTEF1] | Level 0 part |
| 199004 | pGL0_103 [tAgTEF1] | Level 0 part |
| 199005 | pGL0_104 [pAnTrpC] | Level 0 part |
| 199006 | pGL0_105 [tAnTrpC] | Level 0 part |
| 199007 | pGL0_106 [TAmarelleT1] | Level 0 part |
| 199008 | pGL0_107 [TAmarelleT7] | Level 0 part |
| 199009 | pGL0_108 [TAmarelleT9] | Level 0 part |
| 199010 | pGL0_109 [TAmarelleT12] | Level 0 part |
| 199011 | pGL0_110 [pNG2] | Level 0 part |
| 199012 | pGL0_112 [oriT] | Level 0 part |
| 199013 | pGL0_113 [Dummy ORI] | Level 0 part |
| 199014 | pGL0_114 [p15A] | Level 0 part |
| 199015 | pGL0_115 [pBC1 ORI] | Level 0 part |
| 199016 | pGL0_121 [ORI_BC_21] | Level 0 part |
| 199017 | pGL0_122 [ORI_BC_22] | Level 0 part |
| 199018 | pGL0_123 [ORI_BC_23] | Level 0 part |
| 199019 | pGL0_124 [ORI_BC_24] | Level 0 part |
| 199020 | pGL0_125 [ORI_BC_25] | Level 0 part |
| 199021 | pGL0_126 [ORI_BC_26] | Level 0 part |
| 199022 | pGL0_127 [ORI_BC_27] | Level 0 part |
| 199023 | pGL0_128 [ORI_BC_28] | Level 0 part |
| 199024 | pGL0_129 [ORI_BC_29] | Level 0 part |
| 199025 | pGL0_130 [ORI_BC_30] | Level 0 part |
| 199026 | pGL0_142 [EilR-PjexD] | Level 0 part |
| 199027 | pGL0_143 [EilR-PjexH1] | Level 0 part |
| 199028 | pGL0_144 [EilR-PjexH2] | Level 0 part |
| 199029 | pGLEL_4 [BF] | Odd assembly end-linker |
| 199030 | pGLEL_5 [CF] | Odd assembly end-linker |
| 199031 | pGLEL_6 [EF] | Odd assembly end-linker |
| 199032 | pGLDO_1 [α-sfYFP-β] | Level 1 plasmid, methylated YFP dropout |
| 199033 | pGL1_1 | Level 1 plasmid, barcoded ORI |
| 199034 | pGL1_2 | Level 1 plasmid, barcoded ORI |
| 199035 | pGL1_3 | Level 1 plasmid, barcoded ORI |
| 199036 | pGL1_4 | Level 1 plasmid, barcoded ORI |
| 199037 | pGL1_5 | Level 1 plasmid, barcoded ORI |
| 199038 | pGL1_6 | Level 1 plasmid, barcoded ORI |
| 199039 | pGL1_7 | Level 1 plasmid, barcoded ORI |
| 199040 | pGL1_8 | Level 1 plasmid, barcoded ORI |
| 199041 | pGL1_9 | Level 1 plasmid, barcoded ORI |
| 199042 | pGL1_35 | Level 1 plasmid, barcoded ORI |
| 199043 | pGL1_36 | Level 1 plasmid, barcoded ORI |
| 199044 | pGL1_37 | Level 1 plasmid, barcoded ORI |
| 199045 | pGL1_38 | Level 1 plasmid, barcoded ORI |
| 199046 | pGL1_39 | Level 1 plasmid, barcoded ORI |
| 199047 | pGL1_40 | Level 1 plasmid, barcoded ORI |
| 199048 | pGL1_41 | Level 1 plasmid, barcoded ORI |
| 199049 | pGL1_42 | Level 1 plasmid, barcoded ORI |
| 199050 | pGL1_43 | Level 1 plasmid, barcoded ORI |
| 199051 | pGL1_44 | Level 1 plasmid, barcoded ORI |
| 199052 | pGL1_45 | Level 1 plasmid, barcoded ORI |
| 199053 | pGL1_46 | Level 1 plasmid, barcoded ORI |
| 199054 | pGL1_47 | Level 1 plasmid, barcoded ORI |
| 199055 | pGL1_48 | Level 1 plasmid, barcoded ORI |
| 199056 | pGL1_59 | Level 1 plasmid, barcoded ORI |
| 199057 | pGLEL_1 [βω] | Even assembly end-linker |
| 199058 | pGLEL_2 [γω] | Even assembly end-linker |
| 199059 | pGLEL_3 [εω] | Even assembly end-linker |
| 199060 | pGLDV_17 [380G] | Level 0 storage vector with sfGFP dropout for Level 0 parts |
| 199061 | pGLDV_1 [18o1G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199062 | pGLDV_2 [18o2G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199063 | pGLDV_3 [18o3G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199064 | pGLDV_4 [18o4G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199065 | pGLDV_5 [28o1G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199066 | pGLDV_6 [28o2G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199067 | pGLDV_7 [28o3G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199068 | pGLDV_8 [28o4G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199069 | pGLDV_21 [28o1*G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199070 | pGLDV_41 [11o1G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199071 | pGLDV_42 [21o1G] | Odd assembly vector with sfGFP dropout for Odd level assemblies |
| 199072 | pGLDV_9 [18e1G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199073 | pGLDV_10 [18e2G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199074 | pGLDV_11 [18e3G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199075 | pGLDV_12 [18e4G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199076 | pGLDV_13 [38e1G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199077 | pGLDV_14 [38e2G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199078 | pGLDV_15 [38e3G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199079 | pGLDV_16 [38e4G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199080 | pGLDV_19 [11e1G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199081 | pGLDV_20 [31e1G] | Even assembly vector with sfGFP dropout for Even level assemblies |
| 199082 | pGL2_135 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199083 | pGL2_136 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199084 | pGL2_137 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199085 | pGL2_138 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199088 | pGL2_141 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199089 | pGL2_142 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199091 | pGL2_144 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199092 | pGL2_145 | Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion |
| 199093 | pGL2_161 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199094 | pGL2_162 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199095 | pGL2_163 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199096 | pGL2_164 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199097 | pGL2_165 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199098 | pGL2_166 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199099 | pGL2_167 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199100 | pGL2_168 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199101 | pGL2_169 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199102 | pGL2_170 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199103 | pGL2_171 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199104 | pGL2_172 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199105 | pGL2_173 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199106 | pGL2_174 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199107 | pGL2_175 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199108 | pGL2_176 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |
| 199109 | pGL2_177 | Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter |