RIPK3 promoter hypermethylation in hepatocytes protects from bile acid-induced inflammation and necroptosis.
Hoff J, Xiong L, Kammann T, Neugebauer S, Micheel JM, Gassler N, Bauer M, Press AT
Cell Death Dis. 2023 Apr 18;14(4):275. doi: 10.1038/s41419-023-05794-0. PubMed Article
Hoff J, Xiong L, Kammann T, Neugebauer S, Micheel JM, Gassler N, Bauer M, Press AT
Cell Death Dis. 2023 Apr 18;14(4):275. doi: 10.1038/s41419-023-05794-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199313 | pcDNA3-RIPK3[S199A]-FLAG | mutated phosphorylation site (kinase acitivity inhibited) |
| 199315 | pcDNA3-RIPK3[S199D]-FLAG | mutated phosphorylation site (kinase acitivity permanent active) |
| 199318 | pcDNA3-RIPK3[S227A]-FLAG | mutated phosphorylation site (MLKL interaction inhibited) |
| 199320 | pcDNA3-RIPK3[S227D]-FLAG | mutated phosphorylation site (MLKL interaction permanent active) |
| 199323 | pcDNA3-RIPK3[S199A][S227A]-FLAG | double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited) |
| 199324 | pcDNA3-RIPK3[S199D][S227D]-FLAG | double mutated phosphorylation site (kinase acitivity and MLKL interaction active) |
| 199325 | pcDNA3-RIPK3[K5R]-FLAG | mutated ubiquitination site (ubiquitination active) |
| 199326 | pcDNA3-RIPK3[K5A]-FLAG | mutated ubiquitination site (ubiquitination inhibited) |
| 199327 | pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG | mutated RHIM motive (interaction active) |
| 199328 | pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG | mutated RHIM motive (interaction inhibited) |