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Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions.
Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B
J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199446pCA15-Ubc-NLS- scFV-tdTomatoExpression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells
199447pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSDExpession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal
199448pCA17-CRISPR-Tag (11XTS1) for dSpCas9CRISPR-Tag sequence that can be efficiently bound by dSpCas9

Antibodies from Article