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Photodegradable by Yellow-Orange Light degFusionRed Optogenetic Module with Autocatalytically Formed Chromophore.
Chernov KG, Manoilov KY, Oliinyk OS, Shcherbakova DM, Verkhusha VV
Int J Mol Sci. 2023 Mar 30;24(7):6526. doi: 10.3390/ijms24076526. PubMed Article

Plasmids from Article

ID Plasmid Purpose
199463pBAD-degFusionRedArabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.
199464pCMV-Gal4-degFusionRed-p65Expression of the synthetic photodegradable transcription factor Gal4-degFusionRed-p65 in mammalian cells.
199465pcDNA-ALFA-tag-Gal4-VP16Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.
199466pCMV-NbALFA-degFusionRedExpression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

Antibodies from Article