Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome.
Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. PubMed Article
Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ
Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199942 | YP1 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 1 |
| 199943 | YP2 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 2 |
| 199944 | YP3 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 3 |
| 199945 | YP4 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 4 |
| 199946 | YP5 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 5 |
| 199947 | YP6 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 6 |
| 199948 | YP7 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 7 |
| 199949 | YP8 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 8 |
| 199950 | YP9 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 9 |
| 199951 | YP10 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 10 |
| 199952 | YP11 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 11 |
| 199953 | YP12 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 12 |
| 199954 | YP13 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 13 |
| 199955 | YP14 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 14 |
| 199956 | YP15 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 15 |
| 199957 | YP16 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 16 |
| 199958 | YP17 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 17 |
| 199959 | YP18 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 18 |
| 199960 | YP19 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 19 |
| 199961 | YP20 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 20 |
| 199962 | YP21 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 21 |
| 199963 | YP22 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 22 |
| 199964 | YP23 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 23 |
| 199965 | YP24 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 24 |
| 199966 | YP25 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 25 |
| 199967 | YP26 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 26 |
| 199968 | YP27 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 27 |
| 199969 | YP28 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 28 |
| 199970 | YP29 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 29 |
| 199971 | YP30 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 30 |
| 199972 | YP31 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 31 |
| 199973 | YP32 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 32 |
| 199974 | YP33 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 33 |
| 199975 | YP34 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 34 |
| 199976 | YP35 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 35 |
| 199977 | YP36 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 36 |
| 199978 | YP37 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 37 |
| 199979 | YP38 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 38 |
| 199980 | YP39 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 39 |
| 199981 | YP40 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 40 |
| 199982 | YP41 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 41 |
| 199983 | YP42 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 42 |
| 199984 | YP43 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 43 |
| 199985 | YP44 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 44 |
| 199986 | YP45 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 45 |
| 199987 | YP46 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 46 |
| 199988 | YP47 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 47 |
| 199989 | YP48 | Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 48 |
| 199990 | pYP-ptef1-GFP | Yeast/E coli shuttle vector expressing GFP from tef1 promoter |
| 199991 | pT7RNAP-T15 | T7 RNAP circuit variant T15 on miniRK2 plasmid |
| 199992 | pBroad-T7RNAP-chlorR | T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR |
| 199993 | pBroad-T7RNAP-apraR | T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA |
| 199994 | pLP-pTac-Tn5-chlorR | Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information. |
| 199995 | pLP-pTac-himar-apraR | Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below. |
| 199996 | pLP-pR-himar-apraR | Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor |
| 199998 | pInh | Inhibitor of transposase and integrase |
| 199999 | pPath-pTac-integrase | Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below. |
| 200000 | pPath-pR-integrase | Entry vector for biosynthetic pathway. pR-phiC31 integrase |
| 200003 | pPath-Violacein-SGE | Violacein pathway. SGE |
| 200004 | pPath-BGC08-native | BGC08 pathway. Native promoter and CDS sequence |
| 200005 | pPath-BGC08-pT7native | BGC08 pathway. pT7-native CDS sequence |
| 200006 | pPath-BGC08-SGE | BGC08 pathway. SGE |
| 200007 | E. coli MG1655-Landing Pad-Clone 3 | Landing pad (with pIP1433 seed promoter) in MG1655 clone 3 |