Autocatalytic base editing for RNA-responsive translational control.
Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen JX, Chen JC, Vargas-Asencio J, Collins JJ
Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z. PubMed Article
Gayet RV, Ilia K, Razavi S, Tippens ND, Lalwani MA, Zhang K, Chen JX, Chen JC, Vargas-Asencio J, Collins JJ
Nat Commun. 2023 Mar 11;14(1):1339. doi: 10.1038/s41467-023-36851-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200401 | pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen | Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection |
| 200402 | pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES | Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection |
| 200403 | pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES | Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection |
| 200404 | pDV09_pCMV-ADAR1-p110 | Expresses ADAR1 (p110 isoform) constitutively in mammalian cells |
| 200405 | pDV10_pCMV-ADAR1-p150 | Expresses ADAR1 (p150 isoform) constitutively in mammalian cells |
| 200406 | pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES | Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells |
| 200407 | pDV05.0_pEF1a-iRFP720 | Expresses iRFP720 constitutively in mammalian cells |
| 200408 | pDV05.1_pEF1a-iRFP720_frameshifted | Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein |
| 200409 | pDV05.2_pEF1a-iRFP720_secreted | Expresses a secreted version of iRFP720 constitutively in mammalian cells |
| 200410 | pDV06.2c_DART-VADAR_iRFP720-sensor | pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2 |
| 200411 | pDV24_AAV2_DART-VADAR_iRFP720-sensor | AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA |