Chemically Modified mocRNAs for Highly Efficient Protein Expression in Mammalian Cells.
Aditham A, Shi H, Guo J, Zeng H, Zhou Y, Wade SD, Huang J, Liu J, Wang X
ACS Chem Biol. 2022 Dec 16;17(12):3352-3366. doi: 10.1021/acschembio.1c00569. Epub 2022 Jan 7. PubMed Article
Aditham A, Shi H, Guo J, Zeng H, Zhou Y, Wade SD, Huang J, Liu J, Wang X
ACS Chem Biol. 2022 Dec 16;17(12):3352-3366. doi: 10.1021/acschembio.1c00569. Epub 2022 Jan 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200393 | pCS2_hMGFP-60A | A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site. |
| 200394 | pCS2_RFP-60A | Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP. |
| 200395 | pCS2_Firefly_luc-60A | A plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site. |
| 200396 | pCS2_Firefly_degron-60A | A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site. |
| 200397 | pCS2_Renilla_luc-60A | A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site. |