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New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis
Altinoglu I, Carballido-Lopez R
bioRxiv 2023 Article

Plasmids from Article

ID Plasmid Purpose
200418pIA027amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.
200419pIA028thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
200420pIA033thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
200421pIA034amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.
200424pIA030thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
200426pIA035amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Antibodies from Article