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Fluorescent Biosensor for Measuring Ras Activity in Living Cells.
Weeks R, Zhou X, Yuan TL, Zhang J
J Am Chem Soc. 2022 Sep 28;144(38):17432-17440. doi: 10.1021/jacs.2c05203. Epub 2022 Sep 19. PubMed Article

Plasmids from Article

ID Plasmid Purpose
205567pcDNA3.1(+)-RasAR-NESGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.
205568pcDNA3.1(+)-RasAR(R89L)-NESRasAR negative-control mutant plasmid. Targeted to cytosol.
205569pcDNA3.1(+)-PM-RasARPlasma membrane-targeted RasAR.
205570pcDNA3.1(+)-Golgi-RasARGolgi membrane-targeted RasAR.

Antibodies from Article