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ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast.
Akhuli D, Dhar A, Viji AS, Bhojappa B, Palani S
mSphere. 2022 Oct 26;7(5):e0033322. doi: 10.1128/msphere.00333-22. Epub 2022 Oct 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
201764pABY-T1For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast
201765pABY-T2For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast
201766pABY-T3For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast
201767pABY-T4For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast
201768pABY-c15Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast
201769pABY-c25Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast
201771pABY-c45Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast
201774pABY-c46Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Antibodies from Article