Addgene: Applications of an inactive Dispersin B probe to monitor biofilm polysaccharide production.

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Browse
Mark Nitz
Eddenden et al

Applications of an inactive Dispersin B probe to monitor biofilm polysaccharide production.
Eddenden A, Nitz M
Methods Enzymol. 2022;665:209-231. doi: 10.1016/bs.mie.2021.11.006. Epub 2021 Dec 7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
176573	pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis	Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
176575	pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis	Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
176576	pET29b-mVenus-dspB(E184Q)-6xHis	Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
176577	pET29b-mVenus-dspB(E184Q W330Y)-6xHis	Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Antibodies from Article