An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae.
Peng B, Esquirol L, Lu Z, Shen Q, Cheah LC, Howard CB, Scott C, Trau M, Dumsday G, Vickers CE
Nat Commun. 2022 May 24;13(1):2895. doi: 10.1038/s41467-022-30529-8.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185862 | pJCKan | The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker |
| 185863 | pJCHph | The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker |
| 185871 | pILGFP1DFB | A parental plasmid for in vivo gene amplification at RPL25 locus |
| 185872 | pILGFP3A5C | A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (1 copy reference) |
| 185873 | pILGFP3AE4 | A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~11 copy) |
| 185874 | pILGFP3AG4 | A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~12 copy) |
| 185875 | pILGFP3AA5 | A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~47 copy) |
| 185876 | pILGFP3AG4ARSd | A plasmid without an ARS sequence for in vivo gene amplification (HapAmp) at RPL25 locus (~12 copy) |
| 185877 | pILGFP4BG6 | A parental plasmid for in vivo gene amplification (HapAmp) at SEC23 locus |
| 185878 | pILGFP5EG3 | A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (1 copy reference) |
| 185879 | pILGFP5EA4 | A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (4 copy) |
| 185880 | pILGFP5EC4 | A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (9 copy) |
| 185881 | pILGFP5EF3 | A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (13 copy) |
| 185882 | pINER2R | In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (11 copy). |
| 185883 | pINER3R | In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |
| 185884 | pINER4R | In vivo gene amplification (HapAmp) of nerolidol synthetic module locus (25 copy). |
| 185885 | pIT6EG7m | A backbone plasmid for in vivo gene amplification (HapAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy) |
| 185886 | pIT6EG7ml | In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (14 copy). |
| 185887 | pIT6EG7mlh | In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (23 copy). |
| 185889 | pILAC2 | In vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locus |
| 185890 | pILAC3 | In vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locus |
| 185891 | pIAeBlue | In vivo gene amplification (HapAmp) of AeBlue expression module at RPL25 locus |
| 185892 | pIEforRed | In vivo gene amplification (HapAmp) of EforRed expression module at RPL25 locus |
| 185893 | pPAeBlueHPV16LR | 2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus |
| 185894 | pIAeBlueHPV16LR | In vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus |
| 185895 | pINER5 | In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |
| 185896 | pIT7A6 | In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy). |