Expression of the excitatory opsin ChRERalpha can be traced longitudinally in rat and nonhuman primate brains with PET imaging.
Bonaventura J, Boehm MA, Jedema HP, Solis O, Pignatelli M, Song X, Lu H, Richie CT, Zhang S, Gomez JL, Lam S, Morales M, Gharbawie OA, Pomper MG, Stein EA, Bradberry CW, Michaelides M
Sci Transl Med. 2023 Jul 26;15(706):eadd1014. doi: 10.1126/scitranslmed.add1014. Epub 2023 Jul 26. PubMed Article
Bonaventura J, Boehm MA, Jedema HP, Solis O, Pignatelli M, Song X, Lu H, Richie CT, Zhang S, Gomez JL, Lam S, Morales M, Gharbawie OA, Pomper MG, Stein EA, Bradberry CW, Michaelides M
Sci Transl Med. 2023 Jul 26;15(706):eadd1014. doi: 10.1126/scitranslmed.add1014. Epub 2023 Jul 26. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201818 | pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD | A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter |
| 201819 | pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa) | A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter |
| 201820 | pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa) | An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging |
| 201821 | pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa) | An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET |
| 201822 | pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa) | An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging |