Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag.
Liang SQ, Liu P, Ponnienselvan K, Suresh S, Chen Z, Kramme C, Chatterjee P, Zhu LJ, Sontheimer EJ, Xue W, Wolfe SA
Nat Methods. 2023 Jun;20(6):898-907. doi: 10.1038/s41592-023-01859-2. Epub 2023 May 8. PubMed Article
Liang SQ, Liu P, Ponnienselvan K, Suresh S, Chen Z, Kramme C, Chatterjee P, Zhu LJ, Sontheimer EJ, Xue W, Wolfe SA
Nat Methods. 2023 Jun;20(6):898-907. doi: 10.1038/s41592-023-01859-2. Epub 2023 May 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201973 | pET-21a-PE2-His | Express His-tagged PE2 in bacteria |
| 201974 | pegRNA_HEK4_20-7 | Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm |
| 201975 | pegRNA_HEK4_G-T | Express a pegRNA used for G to T conversion at HEK4 site |
| 201976 | pegRNA_HEK4_1bp-deletion | Express a pegRNA used for 1bp deletion at HEK4 site |
| 201977 | pegRNA_HEK4_1bp-insertion | Express a pegRNA used for 1bp insertion at HEK4 site |