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Label-free Single-Molecule Quantification of Rapamycin-induced FKBP-FRB Dimerization for Direct Control of Cellular Mechanotransduction.
Wang Y, Barnett SFH, Le S, Guo Z, Zhong X, Kanchanawong P, Yan J
Nano Lett. 2019 Oct 9;19(10):7514-7525. doi: 10.1021/acs.nanolett.9b03364. Epub 2019 Sep 6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
202380mApple-TalinN10-FRBmApple-Talin(1-1973)-FRB
202381FKBP-TalinC11-mEmeraldFKBP12-Talin(1974-2541)-mEmerald
202382FKBP-TalinRod-mEmeraldFKBP12-Talin(434-2541)-mEmerald
202383FKBP12-talinC11-ABS3mut-mEmeraldFKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald
202384mApple-TalinN3-FRB(wt)mApple-Talin(1-911)-FRB
202385mApple-TalinN12-FB(wt)mApple-Talin(1-2299)-FRB

Antibodies from Article