RB1-deficient prostate tumor growth and metastasis are vulnerable to ferroptosis induction via the E2F/ACSL4 axis.
Wang ME, Chen J, Lu Y, Bawcom AR, Wu J, Ou J, Asara JM, Armstrong AJ, Wang Q, Li L, Wang Y, Huang J, Chen M
J Clin Invest. 2023 Mar 16:e166647. doi: 10.1172/JCI166647. PubMed Article
Wang ME, Chen J, Lu Y, Bawcom AR, Wu J, Ou J, Asara JM, Armstrong AJ, Wang Q, Li L, Wang Y, Huang J, Chen M
J Clin Invest. 2023 Mar 16:e166647. doi: 10.1172/JCI166647. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202511 | pGL3-ACSL4-(-706/-277) | Expressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site) |
| 202512 | pGL3-ACSL4-(-254) | Expressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site) |
| 202513 | pGL3-ACSL4-(-706) | Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site) |
| 202514 | pGL3-ACSL4-(-1899) | Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site) |
| 202515 | pGL3-ACSL4-FL | Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site) |
| 202516 | LentiCRISPR v2 RB1 sgRNA #1 | Expressing Cas9 and sgRNA targeting human RB1 gene |
| 202519 | LentiCRISPR v2 RB1 sgRNA #2 | Expressing Cas9 and sgRNA targeting human RB1 gene |
| 202520 | pCMV-Tag2B-E2F1 | Expressing human E2F1 protein with FLAG-tag |
| 202521 | pCMV-Tag2B-E2F2 | Expressing human E2F2 protein with FLAG-tag |
| 202522 | pCMV-Tag2B-E2F3 | Expressing human E2F3 protein with FLAG-tag |