Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic function.
Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E, Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman JC, Zanivan S, Blyth K, Bryant DM
Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub 2023 Feb 3. PubMed Article
Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E, Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman JC, Zanivan S, Blyth K, Bryant DM
Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub 2023 Feb 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202413 | pQCXIZ GFP PODXL A WT | Expression of GFP-tagged PODXL (isoform A) WT |
| 202414 | pQCXIZ GFP PODXL B WT | Expression of GFP-tagged PODXL (isoform B) WT |
| 202415 | pQCXIZ GFP PODXL delta PBM | Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) |
| 202416 | pQCXIZ GFP PODXL delta PBM + FBM | Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation |
| 202417 | pQCXIZ GFP PODXL FBM | Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin) |
| 202418 | pQCXIZ GFP PODXL delta PBM + Ezrin | Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN |
| 202419 | pQCXIZ GFP PODXL 5N>Q | Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites |
| 202420 | pQCXIZ GFP PODXL 4K>R | Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine) |
| 202421 | pQCXIZ GFP PODXL FKBP 4K>R | Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain |
| 202422 | pQCXIZ GFP PODXL delta IC | Expression of GFP-tagged PODXL without intracellular domain |
| 202423 | pQCXIZ GFP PODXL delta EC | Expression of GFP-tagged PODXL without extracellular domain |
| 202424 | pQCXIZ GFP C1 | Encodes GFP alone |
| 202425 | pQCXIH FRB-BFP | TagBFP FKBP-rapamycin binding (FRB) dimerization domain |
| 202426 | pQCXIH FRB Ubiqutin-BFP | TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin |
| 202428 | pLEX303 TagRFP-T LGALS3 R186S | Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant) |