Condensation of LINE-1 is critical for retrotransposition.
Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ
Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. PubMed Article
Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ
Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202566 | pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2 | Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy |
| 202568 | pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai | Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR |
| 202569 | pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai | Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR |
| 202571 | pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai | Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR |
| 202585 | pETM11-6xHis-TEV-hORF1p_K3AK4A | Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar |
| 202586 | pETM11-6xHis-TEV-hORF1p_R261A | Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar |
| 202587 | pETM11-6xHis-TEV-hORF1p_StammerAAA | Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification |
| 202588 | pETM11-6xHis-TEV-hORF1p_StammerAEA | Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification |