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Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore.
Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, Hummer G
Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. Epub 2024 Feb 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
202777His-ATG3ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop
202778His-ATG3-C264AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop
202779His-ATG3-Y209AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop
202780His-ATG3-Y210AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop
202781His-ATG3-T244AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop
202782His-ATG3-H262AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop
202783His-ATG3-R265AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop
202784His-ATG3-H266AATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop
202785His-ATG3-K208DATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop
202786His-ATG3-K62D/K64DATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Antibodies from Article