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Calling Cards: A Customizable Platform to Longitudinally Record Protein-DNA Interactions Over Time in Cells and Tissues.
Yen A, Mateusiak C, Sarafinovska S, Gachechiladze MA, Guo J, Chen X, Moudgil A, Cammack AJ, Hoisington-Lopez J, Crosby M, Brent MR, Mitra RD, Dougherty JD
Curr Protoc. 2023 Sep;3(9):e883. doi: 10.1002/cpz1.883. PubMed Article

Plasmids from Article

ID Plasmid Purpose
203393pAAV-H2B-tdTomato-SRTAAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards
203394pAAV-rBrokenheartAAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.

Antibodies from Article