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miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells.
Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, Sakurai F, Mizuguchi H
Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
203597CSII-TK-MCSEmpty lentiviral vector to express protein in mammalian cells cells.
203598CSII-TK-B4GALT3Lentiviral vector to express B4GALT3 in mammalian cells
203599CSII-TK-MAIP1Lentiviral vector to express MAIP1 in mammalian cells
203600psicheck2-27bTReporter plasmid carrying miR-27b target sequence
203601psicheck2-B4GALT3Reporter plasmid carrying B4GALT3 3'UTR
203602psicheck2-B4GALT3mutReporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.
203603psicheck2-MAIP1Reporter plasmid carrying MAIP1 3'UTR
203604psicheck2-MAIP1mutReporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences
203605psicheck2-PHLPP2Reporter plasmid carrying PHLPP2 3'UTR
203606psicheck2-PHLPP2mutReporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Antibodies from Article