Addgene: Light-switchable transcription factors obtained by direct screening in mammalian cells.

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Light-switchable transcription factors obtained by direct screening in mammalian cells.
Zhu L, McNamara HM, Toettcher JE
Nat Commun. 2023 Jun 2;14(1):3185. doi: 10.1038/s41467-023-38993-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
203907	pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)	Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells
203908	pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)	Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells
203909	pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)	Encodes wild type Gal4-VP64 transcription factor in mammalian cells
203910	pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)	Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells
203911	pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)	Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Antibodies from Article