Matthias Prigge Lab:
Prigge Lab AAV vectors
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205280 | pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby | Synaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture. |
| 205281 | pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA | CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons. |
| 205282 | pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack | In order to efficiently visualize projection and cell somata in light-sheet preparations, a combination of membrane-targeted and diffusible red fluorophores was developed. Additionally an ALFA tag |
| 205283 | pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE | GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020) |
| 205284 | pAAV_Cbh_DIO_hTyrNull_HATag | Truncated version of human Tyrosinase as control virus for Tyrosinase expression |
| 205285 | pAAV_Ef1a_DIO_hTyrNull_HATag | Truncated version of human Tyrosinase as control virus for Tyrosinase expression |
| 205286 | pAAV_Ef1a_DIO_hTyrHA_minBack | Induces NM-like pigment accumulation in catecholaminergic neurons |
| 205287 | pAAV_Cbh_DIO_hTyr | Induces NM-like pigment accumulation in catecholaminergic neurons |
| 205288 | pAAV_CKII(0.4)_AzPPO_D14_ts_mScarlet_Kv2.1 | Bistable opsin for neuronal inhibition |
| 205289 | pAAV_CKI(0.4)_HA_AzPPO_AgeI_nrxn1a_WT_IRES_mScarlet | Bistable opsin for neuronal inhibition |