A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light.
Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER
bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. Preprint. PubMed Article
Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER
bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. Preprint. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205303 | pRSET-WHaloCaMP1a-EGFP | Expression of WHaloCaMP1a-EGFP fusion in E. coli |
| 205304 | pRSET-WHaloCaMP1a | Expression of WHaloCaMP1a in E. coli |
| 205305 | pRSET-WHaloCaMP1b-EGFP | Expression of WHaloCaMP1b-EGFP fusion in E. coli |
| 205306 | pRSET-WHaloCaMP-eNOSpep-EGFP | Expression of WHaloCaMP-eNOSpep-EGFP fusion in E. coli |
| 205307 | pAAV-synapsin-WHaloCaMP1a-EGFP | Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter |
| 205308 | pAAV-synapsin-WHaloCaMP1a | Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter |
| 205309 | pAAV-CaMKII-WHaloCaMP1a-EGFP | Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter |
| 205310 | pAAV-CaMKII-WHaloCaMP1a | Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter |
| 205311 | pTol2-elavl3-WHaloCaMP1a-EGFP | Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression) |
| 205312 | pTol2-elavl3-WHaloCaMP1a | Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression) |
| 205313 | pCAG-WHaloCaMP1a-EGFP | Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter |
| 205314 | p10xUAS-WHaloCaMP1a-EGFP | Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver |