Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome.
Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670. PubMed Article
Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ
Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206429 | pSAP | A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat). |
| 206431 | pINTO_7/8 | A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome. |
| 206846 | pCHAP1 | A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |
| 206847 | pCHAP2 | A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |
| 206848 | pCHAP3_URA | A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites. |
| 206849 | pCHAP3_Sap | A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment. |
| 206850 | pCHAP4 | A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector. |
| 206851 | pCHAP5 | A plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |
| 206852 | pCHAP6 | A plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |
| 206853 | pCHAP7 | A plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |
| 206855 | pPt_Cp | A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome. |
| 206856 | pPt_Sap | A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome. |
| 206857 | pCHAP8 | A plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1. |