A versatile CRISPR-based system for lineage tracing in living plants.
Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O
Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. PubMed Article
Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O
Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210528 | pGGA-pCLV3 | Cloning A. thaliana CLV3 promoter as GreenGate module A |
| 210533 | pGGB-SpCas9 | Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B |
| 210536 | pGGC-PEA3AT | Cloning P. sativum PEA3A terminator as GreenGate module C |
| 210539 | pGGC-LexACasPea | Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C |
| 210542 | pGGE1-HTR5p | Cloning A. thaliana HTR5 promoter as GreenGate module E1 |
| 210548 | pCLV3Cas-LT0 | Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter |
| 210549 | pCLV3Cas-LT1 | Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter |
| 210550 | pCLV3Cas-LT2 | Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter |
| 210551 | LT0 | Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter |
| 210552 | LT1 | Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter |
| 210553 | LT2 | Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter |
| 210555 | ipGL2Cas | Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter. |
| 210556 | pEF1Cas-LT1 | Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p |
| 210559 | ipYUC2Cas-LT1 | Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter. |