A Validated Set of Ascorbate Peroxidase-Based Organelle Markers for Electron Microscopy of Saccharomyces cerevisiae.
Li H, He CW, Zhu J, Xie Z
mSphere. 2022 Aug 31;7(4):e0010722. doi: 10.1128/msphere.00107-22. Epub 2022 Jun 21. PubMed Article
Li H, He CW, Zhu J, Xie Z
mSphere. 2022 Aug 31;7(4):e0010722. doi: 10.1128/msphere.00107-22. Epub 2022 Jun 21. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207056 | ClhN-4V5-APEX2-URA | For ORF tagging with 4V5-APEX2 |
| 207057 | ClhN-p1k-Pex3-4V5-APEX2-URA | Expressing Pex3-4V5-APEX2. Insufficient signal for EM. |
| 207059 | ClhN-p1k-Tgl3-4V5-APEX2-URA | Expressing Tgl3-4V5-APEX2. Insufficient signal for EM. |
| 207061 | ClhN-p1k-Cox4-V5-APEX2-Ura | Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections. |
| 207062 | ClhN-p1k-Cox4-GFP-APEX2-Ura | Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining. |
| 207063 | ClhN-pADH3-4V5-APEX2-URA | Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag. |
| 207065 | ClhN-pADH3-Snf7-4V5-APEX2-URA | Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional. |
| 207066 | ClhN-pADH3-Tgl3-4V5-APEX2-URA | Expressing Tgl3-4V5-APEX2. Marker for lipid droplets. |
| 207068 | ClhN-pERG6-Pex3-4V5-APEX2-URA | Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes. |
| 207069 | ClhN-pLSP1-Pex3-4V5-APEX2-URA | Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes. |
| 207074 | ClhN-pTPI1-Emc1-GFP-APEX2-Ura | Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining. |
| 207075 | ClhN-pTPI1-Cox4-GFP-APEX2-Ura | Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining. |