REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors.
Boon K, Vanalken N, Meyen E, Schols D, Van Loy T
Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. PubMed Article
Boon K, Vanalken N, Meyen E, Schols D, Van Loy T
Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207148 | pcDNA3.1(+)Gαi1-91-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207149 | pcDNA3.1(+)Gαi2-91-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207150 | pcDNA3.1(+)Gαi3-91-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207151 | pcDNA3.1(+)GαoA-91-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207152 | pcDNA3.1(+)GαoB-91-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207153 | pcDNA3.1(+)Gαq-124-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207154 | pcDNA3.1(+)Gα15-244-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207155 | pcDNA3.1(+)Gα13-125-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2 |
| 207156 | pcDNA3.1(+)Gα12-133-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2 |
| 207157 | pcDNA3.1(+)GαsS-246-Nluc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2 |
| 207158 | pcDNA3.1(+)GαsL-260-NLuc | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2 |
| 207159 | pcDNA3.1(+)Gγ1-LSS-mKATE2(N) | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA |
| 207160 | pcDNA3.1(+)Gγ2-LSS-mKATE2(N) | Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA |