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Isosteric Engineering of Enzymes: Overcoming Activity-Stability Trade-Offs by Site-Selective CH --> N Substitutions.

Abdelkader EH, Qianzhu H, Otting G, Huber T
Angew Chem Int Ed Engl. 2026 Jul 13:e8993497. doi: 10.1002/anie.8993497. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207617pRSF-G1(4AW)RStRNA synthetase/tRNA pair for the in vivo incorporation of 4-Aza-L-Tryptophan (4AW) into proteins in E. coli in response to the amber (TAG) codon
207618pRSF-G1(5AW)RStRNA synthetase/tRNA pair for the in vivo incorporation of 5-Aza-L-Tryptophan (5AW) into proteins in E. coli in response to the amber (TAG) codon
207619pRSF-G1(6AW)RStRNA synthetase/tRNA pair for the in vivo incorporation of 6-Aza-L-Tryptophan (6AW) into proteins in E. coli in response to the amber (TAG) codon

Antibodies from Article