Proximity proteomic analysis of the NRF family reveals the Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor of NRF2.
LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, Ashrafi G, Ben Major M
Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 10.1126/scisignal.adi9018. Epub 2023 Dec 12. PubMed Article
LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, Ashrafi G, Ben Major M
Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 10.1126/scisignal.adi9018. Epub 2023 Dec 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207809 | p663-UBC-miniTurbo-V5-NRF1 | N-terminal tagged NRF1 for miniTurbo proximity experiments |
| 207810 | p667-UBC-NRF1-V5-miniTurbo | C-terminal tagged NRF1 for miniTurbo proximity experiments |
| 207811 | p663-UBC-miniTurbo-V5-NRF2 | N-terminal tagged NRF2 for miniTurbo proximity experiments |
| 207812 | p667-UBC-NRF2-V5-miniTurbo | C-terminal tagged NRF2 for miniTurbo proximity experiments |
| 207813 | p663-UBC-miniTurbo-V5-NRF2-E79Q | N-terminal tagged NRF2-E79Q for miniTurbo proximity experiments |
| 207814 | p667-UBC-NRF2-E79Q-V5-miniTurbo | C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments |
| 207816 | p667-UBC-NRF3-V5-miniTurbo | C-terminal tagged NRF3 for miniTurbo proximity experiments |
| 207817 | p663-UBC-miniTurbo-V5-MAFG | N-terminal tagged MAFG for miniTurbo proximity experiments |
| 207818 | p667-UBC-MAFG-V5-miniTurbo | C-terminal tagged MAFG for miniTurbo proximity experiments |
| 207819 | p663-UBC-miniTurbo-V5-ZNF746 | N-terminal tagged ZNF746 for miniTurbo proximity experiments |
| 207820 | p667-UBC-ZNF746-V5-miniTurbo | C-terminal tagged ZNF746 for miniTurbo proximity experiments |
| 207821 | p663-UBC-miniTurbo-V5-ZNF746-C571A | N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments |
| 207822 | pLenti-Cas9-ZNF746 | Dual expression of Cas9 and sgRNA targeting ZNF746 |
| 207823 | pLenti-Cas9-NS-A | Dual expression of Cas9 and non-specific sgRNA |
| 207824 | pLenti-Cas9-NS-B | Dual expression of Cas9 and non-specific sgRNA |
| 207825 | pCW57.1-ZNF746 | Dox-inducible expression of ZNF746 |
| 207826 | pCW57.1-ZNF746-C571A | Dox-inducible expression of ZNF746-C571A |
| 207827 | pHAGE-PGUS | FLAG-tagged PGUS driven by CMV promoter |
| 207828 | pHAGE-NRF2-E79Q | FLAG-tagged NRF2-E79Q driven by CMV promoter |