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A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks.
English MA, Alcantar MA, Collins JJ
Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 10.15252/msb.202211398. Epub 2023 Mar 27. PubMed Article

Plasmids from Article

ID Plasmid Purpose
213902pDonor_Tn[sfGFP] Mariner transposon expressing sfGFP with kan selection casette
213903pDonor_Tn[GFP] Mariner transposon expressing mNeonGreen with kan selection casette
213904pDonor_Tn[RFP] Mariner transposon expressing mCherry with cam selection casette
213905pDonor_Tn-pOUT Mariner transposon with outward-facing pJ23104 promoter
213906pDonor_Tn[himar1C9] Mariner transposon with ATc-inducible himar1C9 transposase
213907pDonor_RB-TnV2 Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
213908pDonor_RB-TnV2_pJEx Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites
213909pHelper_AraC/pBad-Tnpase (camR)Arabinose-inducible himar1C9 transposase expression - cam resistance
213910pHelper_TetR/pTet-Tnpase (camR) ATc-inducible himar1C9 transposase expression - cam resistance
213911pHelper_TetR/pTet-Tnpase (carbR) ATc-inducible himar1C9 transposase expression - carb/amp resistance

Antibodies from Article