MRE11 liberates cGAS from nucleosome sequestration during tumorigenesis.

Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis JE, Gupta GP
Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
208382	Lenti-sgRNA-Cre-GpNLuc	Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.
208383	Lenti-sgRNA-Cre-GpNluc-sgControl	This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.
208384	Lenti-sgRNA-Cre-GpNLuc-sgmMRE11	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.
208385	Lenti-sgRNA-Cre-GpNLuc-sgmcGAS	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.
208386	Lenti-sgRNA-Cre-GpNLuc-sgmSTING	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.
208387	Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.
208388	Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.
208389	Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.
208391	Lenti-sgRNA-Cre-GpNLuc-sgmMLKL	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MLKL gene.
208392	Lenti-sgRNA-Cre-GpNLuc-sgmATM	Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.
208393	pBABE-puro-Halo-hMRE11(wt)	To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.
208394	pBABE-puro-Halo-hMRE11(H129N)	To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.
208395	pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)	To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.
208396	PB-TA-ERP2-hcGAS(wt)	To generate stable cell line expressing human cGAS(wt) using PiggyBac transposon system
208397	PB-TA-ERP2-hcGAS(R255A)	To generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system