A Fluorescence-Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants.

Haas BE, Saif F, Bolger E, Doran L, Rosales E, Kim A, Burgess SJ, Long SP
Plant Direct. 2026 Apr 15;10(4):e70163. doi: 10.1002/pld3.70163. eCollection 2026 Apr. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
209019	RC00214-pcsb-35Sp-ccdB-mNG-OCSp-tdT	Dual-fluorescence acceptor plasmid for one-step insertion of a 5' leader of interest between the CaMV35S promoter and mNeonGreen via BpiI. A tdTomato expression cassette serves as internal control.