Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells.
Sekhon H, Ha JH, Presti MF, Procopio SB, Jarvis AR, Mirsky PO, John AM, Loh SN
Nat Methods. 2023 Nov 9. doi: 10.1038/s41592-023-02065-w. PubMed Article
Sekhon H, Ha JH, Presti MF, Procopio SB, Jarvis AR, Mirsky PO, John AM, Loh SN
Nat Methods. 2023 Nov 9. doi: 10.1038/s41592-023-02065-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209702 | pCMV-RBP-WDR5 | Mammalian expression of WDR5 with N terminal RBP fusion |
| 209703 | pCMV-mCherry(Y70A) | Mammalian expression of mCherry with Y70A mutation |
| 209704 | pCMV-SH2 | Mammalian expression of SH2 |
| 209705 | pCMV-HRas(G12V) | Mammalian expression of HRas with G12V mutation |
| 209706 | pCMV-MBP-yATOM_WDR5 | Mammalian expression of y-ATOM biosensor for WDR5 |
| 209707 | pCMV-MBP-yATOM_mCherry | Mammalian expression of y-ATOM biosensor for mCherry |
| 209708 | pCMV-MBP-yATOM_SH2 | Mammalian expression of y-ATOM biosensor for SH2 |
| 209709 | pCMV-MBP-yATOM_Ras | Mammalian expression of y-ATOM biosensor for HRas |
| 209710 | pCMV-NLS-MBP-rATOM(WDR5) | Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal |
| 209711 | pCMV-NES-MBP-cATOM(Ras) | Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal |
| 209712 | pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX | Mammailan expression of WDR5, SH2, and HRas separated by P2A. |
| 209713 | pCMV-MBP-yATOM_WDR5 (R80A) | Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation |
| 209714 | pCMV-PRLSS-yATOM_SH2-KDEL | Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum |
| 209715 | pCMV-Cox4-yATOM_SH2 | Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria |
| 209716 | pCMV-PRLSS-SH2-KDEL | Mammalian expression of SH2 localized to endoplasmic reticulum |
| 209717 | pCMV-Cox4-SH2 | Mammalian expression of SH2 localized to mitochondria |
| 209718 | pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL | Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum |
| 209719 | pCMV-Cox4-NbGFP_miRFP670nano3 | Mammalian expression of NbGFP-miRFP670nano3 localized to mitochondria |
| 209720 | pCMV-PRLSS-Clover-KDEL | Mammalian expression of clover localized to endoplasmic reticulum |
| 209721 | pCMV-Cox4-Clover | Mammalian expression of clover localized to mitochondria |