Protocol for allele specific epigenome editing using CRISPR/dCas9
Rajaram N, Bashtrykov P, Jeltsch A
Methods Mol Biol, in press.
Rajaram N, Bashtrykov P, Jeltsch A
Methods Mol Biol, in press.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210212 | sgRNA_expression_vector | An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI. |