ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization.
Nakamura H, Rho E, Lee CT, Itoh K, Deng D, Watanabe S, Razavi S, Matsubayashi HT, Zhu C, Jung E, Rangamani P, Watanabe S, Inoue T
Cell Rep. 2023 Sep 2:113089. doi: 10.1016/j.celrep.2023.113089.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210289 | Tom20-ECFP-FRB | mitochondrial OM marker and "anchor" in FKBP-FRB CID |
| 210290 | ECFP-FRB-Sec61b | nucleus OM marker and "anchor" for FKBP-FRB CID |
| 210291 | Tom20-EYFP-miLID | mitochondria marker and "anchor" for optogenetics |
| 210292 | EYFP-Parkin | mitophagy marker |
| 210293 | Lifeact-EYFP | marker for F-actin filaments |
| 210294 | mActA-mSspB-mCherry | ActuAtor for iLID optogenetics |
| 210295 | mActA-FKBP-mCherry | ActuAtor for FKBP-FRB CID |
| 210296 | ECFP-FRB-GiantinS | Golgi marker and anchor for FKBP-FRB CID |
| 210299 | EYFP-FKBP-Nck-SH3 | targeted actin nucleation |
| 210301 | EYFP-FKBP-DN-N-WASP | control for targeted actin nucleation |
| 210302 | EYFP-FKBP-N-WASP | targeted actin nucleation |
| 210303 | ECFP-FRB-G3BP | stress granule anchor and marker |
| 210304 | ECFP-FRB-TIA1 | stress granule anchor and marker |
| 210305 | ECFP-FRB-FXR1B | stress granule anchor and marker |