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Massively parallel in vivo Perturb-seq reveals cell type-specific transcriptional networks in cortical development.
Zheng X, Wu B, Liu Y, Simmons SK, Kim K, Clarke GS, Ashiq A, Park J, Wang Z, Tong L, Wang Q, Xu X, Levin JZ, Jin X
bioRxiv. 2023 Sep 18:2023.09.18.558077. doi: 10.1101/2023.09.18.558077. Preprint. PubMed Article

Plasmids from Article

ID Plasmid Purpose
210523pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IRAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.
210525pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IRAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.
213480pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IRAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.
213481pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IRAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Antibodies from Article