Amber Suppressor tRNA-Based Mutagenesis for Positional Semi-Saturated Mutagenesis with Natural Amino Acid Substitutions: An Approach for Mapping Positional Contributions to Protein Function
O’Neil PT, Zeczycki TN, Park K-T, Rodnin MV, Swint-Kruse L, Vincentelli R, Fenton AW
Biophysica. 2026; 6(4):72.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211556 | pAla57 | Amber Suppressor tRNA for Alanine |
| 211557 | pArg57 | Amber Suppressor tRNA for Arginine |
| 211558 | pCys57 | Amber Suppressor tRNA for Cysteine |
| 211559 | pGln57 | Amber Suppressor tRNA for Glutamine |
| 211560 | pGlu57 | Amber Suppressor tRNA for Glutamate |
| 211561 | pGly57 | Amber Suppressor tRNA for Glycine |
| 211562 | pHis57 | Amber Suppressor tRNA for Histidine |
| 211563 | pLeu57 | Amber Suppressor tRNA for Leucine |
| 211564 | pLys57-1 | Amber Suppressor tRNA for Lysine |
| 211565 | pLys57-2 | Amber Suppressor tRNA for Lysine (Methionine-derived) |
| 211566 | pLys57-3 | Amber Suppressor tRNA for Lysine (Isoleucine-derived) |
| 211567 | pLys57-4 | Amber Suppressor tRNA for Lysine (Aspartate-derived) |
| 211568 | pPhe57 | Amber Suppressor tRNA for Phenylalanine |
| 211569 | pPro57 | Amber Suppressor tRNA for Proline |
| 211570 | pSer57 | Amber Suppressor tRNA for Serine |