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Coupling during collective cell migration is controlled by a vinculin mechanochemical switch.
Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 10.1073/pnas.2316456120. Epub 2023 Dec 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
211835pRRL-VinculinTS-S1033AVinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
211893pRRL-VinculinTS-S1033DVinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
211894pRRL-VinculinCS-S1033AVinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
211895pRRL-VinculinCS-S1033DVinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
211896pRRL-VinculinVenus-S1033AVinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.
211897pRRL-VinculinVenus-S1033DVinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

Antibodies from Article